TABLE 4.
Primers used to generate plasmid constructs
| Name | Sequence (5′→3′) | Size (bp) | Plasmid constructa |
|---|---|---|---|
| pyk_F_NdeI | GGAATTCCATATGATGGGCGTGGATAGACGAAC | 1,434 | pCRE555 |
| pyk_R_NdeI | GGAATTCCATATGTTAGAGCTTTGCAATCCTTGTG | ||
| pfk_ F_NdeI | GGAATTCCATATGATGGAAGACATGCGAATTGCTAC | 1,041 | pCRE557 |
| pfk_ R_NdeI | GGAATTCCATATGCTATCCAAACATTGCCTGGG | ||
| Ptac_KpnI | CGGGGTACCGGCTGTGCAGGTCGTAAATC | 1,704 | pCRE558 |
| rrnB_KpnI | CGGGGTACCAAAAAGGCCATCCGTCAGGA | ||
| CglR2326-Fw5(SalI) | CTCTGTCGACTGATTTCATCGCGCTGGTTG | 1,119 | pCRE554 |
| CglR2326-Rv6 | GCATGGGCGACCTATGG | ||
| CglR2326-Fw6 | ATCGCTACTATTGTTTCCATAGGTCGCCCATGCGACCGCCTCATCCTCGT | 1,008 | |
| CglR2326-Rv5(SalI) | CTCTGTCGACGAATGAAACTTCTCACCCGTG | ||
| CglR0733-Fw1(SphI) | CTCTGCATGCACTGCGTAGGTCGATTTCC | 1,048 | pCRE553 |
| CglR0733-Rv2 | CTCCCATAGTGGTAGACG | ||
| CglR0733-Fw2 | CCAGGGGAGCCGTCTACCACTATGGGAGACTCCCTGAACTGGTCTTTTAG | 1,041 | |
| CglR0733-Rv1(SalI) | CTCTGTCGACCTTACTTCTTGTACCCGAGC | ||
| indel10- Fw4(XbaI) | CTCTTCTAGAACACACCTCATCACGAGTAG | 15,739 | pCRE552 |
| indel10-Rv2(XbaI) | CTCTTCTAGACCTCGTCAGTTGCTGCAGTT | ||
| 0035-XbaI-F | GCTCTAGAGGCACTGAGCTTTGCAGCTA | 1,631 | pCRE500 pCRE501 |
| 0035-SalI-R | ACGCGTCGACCCCCGCAACGCATCATTAAC | ||
| 0141-SalI-F | TATGTCGACATGCTCGAAGGCATCATCTACC | 1,600 | pCRE502 pCRE503 |
| 0141-SalI-R | TATGTCGACATCGAACCATCATCGTGTTGGA | ||
| 0489-XbaI-F | GCTCTAGAACTGCACATTGTGACCACCC | 940 | pCRE504 pCRE505 |
| 0489-SalI-R | ACGCGTCGACGATTTTTGCACCACAGGCCG | ||
| 1044-XbaI-F | GCTCTAGACCAAAAACGTCATGGCAGGC | 2,072 | pCRE506 pCRE507 |
| 1044-SalI-R | ACGCGTCGACGAAATCGCGTGCCGTCATTG | ||
| 1064-SalI-F | TATGTCGACATCTCGCTCATAGATTCCACGG | 1,100 | pCRE508 pCRE509 |
| 1064-SalI-R | TATGTCGACACTTGCCAGAAATCCCGCTGTA | ||
| 1240-SalI-F | TATGTCGACATACCTGCTGCTACTTGTTGTG | 1,000 | pCRE510 pCRE511 |
| 1240-SalI-R | TATGTCGACAAGATCTCAAGGAACTCGCTTC | ||
| 1294-SalI-F | TATGTCGACAAGGCATGATTCAGCTCATCGC | 1,100 | pCRE512 pCRE513 |
| 1294-SalI-R | TATGTCGACAAGCCACTGAACTGGATGACAC | ||
| 2048-XbaI-F | GCTCTAGAACTCGATGACAAAGGCAGGC | 2,559 | pCRE517 pCRE518 |
| 2048-SalI-R | ACGCGTCGACTCTCTAGCGGCTCTTCTGCT | ||
| 2057-SalI-F | TATGTCGACAGGACAATCAGATTGTGGGCAC | 1,000 | pCRE519 pCRE520 |
| 2057-SalI-R | TATGTCGACACGAACGGTGCTTAAGGATCGA | ||
| 2303-SalI-F | TATGTCGACACAATGACTCTCCGCATTGGTC | 1,000 | pCRE524 pCRE525 |
| 2303-SalI-R | TATGTCGACATTGTCACGTTGCTTTCAGCGC | ||
| 2333-XbaI-F | GCTCTAGAACTGGGGAAAGTGGGGAAATG | 854 | pCRE526 pCRE527 |
| 2333-SalI-R | ACGCGTCGACAGCGGCGCTGTATAAGAAGC | ||
| 2531-SacI-F | TGCGAGCTCGTTGCTAACCGTCTGCCAGT | 1,721 | pCRE528 pCRE529 |
| 2531-SalI-R | ACGCGTCGACAGACAAGGTCACATAATCGGCA | ||
| 2654-XbaI-F | GCTCTAGAACTGCAGTACATGCCTGAGGAA | 1,560 | pCRE533 pCRE534 |
| 2654-XbaI-R | GCTCTAGAATTGGCGGTATTGCTCGTCTTC | ||
| 2975-XbaI-F | GCTCTAGATGTTTATCCCGCCAGGAAGC | 997 | pCRE535 pCRE536 |
| 2975-SalI-R | ACGCGTCGACAGTGAACACCTCACCGTGGA | ||
| 1704-SalI-F | TATGTCGACATTGCTAACCGACTGCTTCGTC | 1,000 | pCRE514 pCRE515 pCRE516 |
| 1704-SalI-R | TATCGATCGATCAACCAGACAACCACGCTTG | ||
| 2163-SalI-F | TATGTCGACAACAGAGATGCTGACC | 1,500 | pCRE521 pCRE522 pCRE523 |
| 2163-SalI-R | TATGTCGACAACAATGCGGCGCTTA | ||
| 2547-XbaI-F | GCTCTAGACGCAAAATCCCTTGATCGGACA | 2,094 | pCRE530 pCRE531 pCRE532 |
| 2547-SalI-R | ACGCGTCGACTTATGGTGTTGCTGGCACCG | ||
| 1015-XbaI-F | GCTCTAGAACCGGCTATTTTACCTCTGC | 1,523 | pCRE537 pCRE538 |
| 1016-SalI-R | ACGCGTCGACTGTGTAGCGGAATG | ||
| 1377-XbaI-F | GCTCTAGATTAATTGTTCTCGTCGTTGC | 1,172 | pCRE539 pCRE540 |
| 1378-SalI-R | ACGCGTCGACATGTGATGGGCAACTAATCT | ||
| 1414-SacI-F | AGCGAGCTCTGGAAGCCACTAGCACTCAT | 1,633 | pCRE541 pCRE542 |
| 1415-SalI-R | ACGCGTCGACCAAGATATTCGACGAGGGCG | ||
| 1462-SacI-F | AGCGAGCTCCTTGACCGATGATCTGCACC | 2,358 | pCRE543 pCRE544 |
| 1463-XbaI-R | GCTCTAGAAGCCAGGTTGAAGTCGTACA | ||
| 2959-XbaI-F | GCTCTAGAGTTCGGCCCAATCCTGTTCT | 1,953 | pCRE545 pCRE546 |
| 2960-SalI-R | ACGCGTCGACTGGGATACGAGTACGCAGGT |
a
Described in Table 3.