TABLE 5.

Growth properties of the parental and evolved strains

Strain	Nutrient-rich medium			Minimal medium
	Tmaxa (°C)	Maximal growth rate (h−1)b		Tmax (°C)	Maximal growth rate (h−1)b
		33°C	41°C		33°C	38°C
GLY3	40.2	0.88 ± 0.041	0.14 ± 0.063	37	0.57 ± 0.050	0.26 ± 0.021
trm2	41.5	0.72 ± 0.014c	0.33 ± 0.041c	40.5	0.55 ± 0.0099	0.38 ± 0.022c
tam44	41.2	0.66 ± 0.020c	0.29 ± 0.025c	40	0.55 ± 0.040	0.38 ± 0.035c
tam45	41.5	0.70 ± 0.056c	0.33 ± 0.0091c	40.5	0.55 ± 0.024	0.39 ± 0.013c

^aAt temperatures higher than the T_max, growth of the strain never reaches an OD₆₁₀ of 1.0 within 24 h.

^bMeans ± standard deviations (n = 3).

^cA statistically significant difference was observed compared to GLY3 (P < 0.01).