TABLE 6.
Amino acid substitutions common to the evolved strains
| Position, nt [strain(s)] | Gene |
Protein | Mutation |
Thermotolerancea |
|||
|---|---|---|---|---|---|---|---|
| Locus tag | Name | mRNA | Protein | Introduction | Reversion | ||
| 40393 | CgR_0035 | Putative glycosyltransferase | 1043C→T | G348D | ± | ± | |
| 157961 | CgR_0141 | Transposase | 470A→G | V157A | ± | ± | |
| 545579 | CgR_0489 | hemC | Porphobilinogen deaminase | 574G→A | A192T | ± | ± |
| 1152246 | CgR_1044 | glmU | Bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase | 883C→T | E295K | + | ± |
| 1179478 | CgR_1064 | Putative esterase | 767G→A | A256V | ± | ± | |
| 1356940 | CgR_1240 | mhpA | 3-(3-Hydroxyphenyl)propionate hydroxylase | 1074G→A | M358I | ± | ± |
| 1423519 | CgR_1294 | nucS | Endonuclease | 482G→A | R161H | ± | ± |
| 2258272 | CgR_2048 | mraW | S-Adenosyl-methyltransferase | 848G→A | P283L | ± | ± |
| 2267850 | CgR_2057 | pknL | Serine/threonine protein kinase | 1099A→G | T367A | ± | ± |
| 2538467 | CgR_2303 | Putative ketosteroid isomerase | 497C→T | P166L | ± | ± | |
| 2567418 | CgR_2333 | Putative metal-dependent hydrolase | 566A→G | Q189R | ± | ± | |
| 2798011 | CgR_2531 | otsA | Trehalose-6-phosphate synthase | 983G→A | R328H | + | − |
| 2936712 | CgR_2654 | pknG | Serine/threonine protein kinase | 431A→G | D144G | ± | ± |
| 3297495 | CgR_2975 | Putative NUDIX hydrolase | 185A→G | Q62R | ± | ± | |
| CgR_2976 | Hypothetical protein | 161T→C | L54P | ||||
| 1899467 (trm2, tam44) | CgR_1704 | secNb | Preprotein translocase | 308T→C | D103G | ± | ± |
| 1899707 (tam45) | 68C→T | R23H | ± | ± | |||
| 2385598 (tam45) | CgR_2163 | hrcAb | Heat-inducible transcription repressor | 994C→T | A332T | ± | ± |
| 2386507 (trm2, tam44) | 85A→G | S29P | ± | ± | |||
| 2813228 (tam45) | CgR_2547 | ptsSb | PTS sucrose-specific transporter | 1019G→A | S340F | ± | ± |
| 2813766 (trm2, tam44) | 481G→A | L161F | ± | ± | |||
a
Mutations were introduced into GLY3 (Introduction) or replaced by the wild-type genes in the evolved strains (Reversion). ±, unchanged; +, increased; −, decreased.
b
Mutations in these genes occurred at different positions in tam45 than in the other two evolved strains.