TABLE 2.
Primers and probes
| Primer or probea | Sequence 5′→3′b | Amplicon size (bp) |
|---|---|---|
| fliC-I_F | ACATGAGTGCTAAATCTTTATCAG | 122 |
| fliC-I_R | GTTAGCTCTTTCTGCTGATAC | |
| fliC-I_P | TTGCTGCTGAACAAACCTTAGCACTCT | |
| fliC-II_F | ATCAGCTTCAAGCATAGAC | 111 |
| fliC-II_R | CTAACTTTAGCTGCATCAAC | |
| fliC-II_P | AAGCAGTGGCTCTCATATCACCAATT | |
| fliC-III_F | AGCAAACAAAGATCAAAACATCAC | 106 |
| fliC-III_R | CTGCTGAACATTTACTCGCAC | |
| fliC-III_P | ACAGATGCTACCTTTAATGCTGAAGCACT | |
| fliC-IV_F | AAAGATGCTGCTTTAACTGG | 117 |
| fliC-IV_R | ATCACCACTAACTTCTACTGC | |
| fliC-IV_P | TAATTCCATCTGCACCATCAGCTGTTAT | |
| fliC-V_F | AATCAATGGGCGCAACAGGA | 141 |
| fliC-V_R | GAACTGATCCTAAATCTGATCTG | |
| fliC-V_P | TGAGCATTTGTTGCATCTTTACCAGAAACA | |
| Novyi-sensu-lato_F | GGAACCAACCTACCGAG | 100 |
| Novyi-sensu-lato_R | GTAGCCACCTCCTTAACA | |
| Novyi-sensu-lato_P | TCCAGACACGAAGAGGCTTAATATATCCA | |
| ntnh-grpIII_F | TACTGATTTATTTAGACCTGATTG | 122 |
| ntnh-grpIII_R | CTTTAGGCACCTTGTTATTG | |
| ntnh-grpIII_P | TCATCCAATTATAATCTCCATCTCTAAGTGA |
a
F, forward (primer); R, reverse (primer); P, probe.
b
All probes were labeled with 6-HEX or 6-FAM and BHQ1 (Black Hole Quencher).