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The pH-dependent activities (kcat) of Zn-, Cu-, and Co-MolA on p-nitrophenyl butyrate, from which dissociation constants were generated. The activities of Zn-MolA (A; pKa1, 6.73; pKa2, 9.36), Cu-MolA (B; pKa1, 5.56; pKa2, 7.56), and Co-MolA (C; pKa1, 6.45; pKa2, 9.50) on p-nitrophenyl butyrate are shown. Points are averages of three replicates, and error bars represent the standard deviations of three independent replicates.
