FIG 7.
Kinetic parameters of variants used to probe the structural determinants of MolA substrate specificity. (A) kcat values of variants relative to those of wild-type (WT) MolA with the substrates molinate (Mol), ethiolate (Eth), paraoxon ethyl (PE), l-alanine p-nitroanilide (APN), and p-nitrophenyl butyrate (PNB). (B) Km values of variants relative to those of the wild type with the same substrates. (C) kcat/Km ratios of variants relative to those of the wild type. (D) Positions of the respective mutations (green) in the structure of MolA docked with molinate (yellow) in AutoDock (44). Full kinetic parameters are shown in Table S2 in the supplemental material.