TABLE 1 .
Peptide mass fingerprinting identifications
| Band no.a | Protein(s) identifiedb | Class |
|---|---|---|
| 1 | NTHI1164 IgA-specific serine endopeptidase | Autotransporter |
| 2 | NTHI0354 adhesion and penetration protein Hap | Autotransporter |
| 3 | NTHI0782 hemoglobin-haptoglobin binding protein B | OMP |
| NTHI1450 HMW2A (high-molecular-weight adhesin 2) | Autotransporter | |
| 4 | NTHI0371 heme/hemopexin binding protein A | Autotransporter |
| NTHI1390 heme utilization protein | OMP | |
| 5 | NTHI1905 periplasmic serine protease HtrA* | Periplasmic |
| 6 | NTHI0522 OmpP1 long-chain fatty acid ABC transporter | OMP |
| 7 | NTHI0481 periplasmic chelated iron binding protein (protein F)* | Periplasmic |
| NTHI0588 survival protein SurA-like protein* | Periplasmic | |
| 8 | NTHI1332 OmpP5 (outer membrane protein P5) | OMP |
| 9 | NTHI1083 outer membrane protein 26* | Periplasmic |
| 10 | NTHI0353 hypothetical protein | Lipoprotein |
| NTHI0501 outer membrane protein P6 | Lipoprotein |
a
The band number was assigned as shown in the SDS-polyacrylamide gel in Fig. 6A. Bands 1 to 9 represented proteins with different staining intensities in the 176 wt and 176ΔenvC OMVs. Band 10 corresponded to proteins with similar staining intensities.
b
The proteins that are less abundant in the 176ΔenvC strain compared to the 176 wt strain are indicated by an asterisk.