TABLE 1 .
qPCR primer sequences
| RNA source | Gene producta | Direction | Nucleotide sequence |
|---|---|---|---|
| Rabbit | IL-6 | Forward | GTCAACTGCATGAACAGAAAGG |
| Reverse | AGCAGGCAGGTCTCATTATTC | ||
| IL-8 | Forward | CCTGCTGTCTCTGACTCTTTG | |
| Reverse | AGGTGTGGAGTGTGTCTTTATG | ||
| TNF-α | Forward | CCCAAACAACCTCCATCTAGTC | |
| Reverse | CACTTGCGGGTTTGCTACTA | ||
| MCP-1 | Forward | AGTCACCTGCTGCTATACATTC | |
| Reverse | ACAGCTTCTTTGGGACACTT | ||
| Adiponectin | Forward | TCCTACCACATCACCGTCTAT | |
| Reverse | CACGTTCTTGTCCTGGTACTG | ||
| PPARγ | Forward | CCGAGAAGGAGAAGCTGTTG | |
| Reverse | CGGGAAGGACTTGATGTATGAG | ||
| Insulin | Forward | AGCTGGCCCTGCAGAAG | |
| Reverse | CCCTAGTTGCAGTAGTTCTCC | ||
| HDAC9 | Forward | GGCTGTGAAGATAAAGGAGGAA | |
| Reverse | AGGAAGGGCTGTTGCATAAA | ||
| GAPDH | Forward | GACCACTTTGTGAAGCTCATTTC | |
| Reverse | GTGGTTTGAGGGCTCTTACTC | ||
| HSL | Forward | CTAGCTCAACAGGAAGCTGAA | |
| Reverse | GCTCTCTCTTGGGATGTAGATTG | ||
| Human | ATGL | Forward | TGTCTGCAGCGGTTTCAT |
| Reverse | CTCATAGAGTGGCAGGTTGTC | ||
| HSL | Forward | GATGGAAGTGCTATCGTCTCTG | |
| Reverse | AGTCAGTGGCATCTCAAAGG | ||
| MGL | Forward | AGGACACCAGACTGCAATAAC | |
| Reverse | ACAGCAACCACCTCATCAC | ||
| GAPDH | Forward | GGTGTGAACCATGAGAAGTATGA | |
| Reverse | GAGTCCTTCCACGATACCAAAG |
a
MCP-1, monocyte chemoattractant protein-1; PPARγ, peroxisome proliferator-activated receptor gamma; HDAC9, histone deacetylase-9; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HSL, hormone-sensitive lipase; ATGL, adipose triglyceride lipase; MGL, monoglyceride lipase.