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. 2015 Mar 3;6(2):e02507-14. doi: 10.1128/mBio.02507-14

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Copyright © 2015 Renzi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — Ccan_15070 encodes an N-acetylglucosamine-1-P uridyltransferase. (A) Schematic representation of the C. canimorsus 5 Ccan_15070 gene-encoded N-acetylglucosamine-1-P-uridyltransferase (Uniprot ID F9Yr13). (B) Growth on HEK293 cells of wt (C. canimorsus 5), PUL5 (ΔPUL5), and PUL5/Ccan_15070 (ΔPUL5/ΔCcan_15070) double-mutant bacteria expressing GlmM and GlmU (MOI, 0.05; 23 h of growth). The averages from three independent experiments are shown. Error bars represent 1 standard deviation from the mean. (C) Bright-field microscopy picture of PUL5 Ccan_15070 double mutant bacteria expressing GlmM and GlmU grown for 23 h on HEK293 cells (MOI, 0.05). (D) Detection of UDP-GlcNAc production by HPLC. Incubation of recombinant Ccan_15070 with UTP and GlcNAc-1-P resulted in the production of UDP-GlcNAc. (E) No UDP-GlcNAc formation could be detected in the absence of recombinant Ccan_15070.