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. 2015 Jan 7;92(3):67. doi: 10.1095/biolreprod.114.125708

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© 2015 by the Society for the Study of Reproduction, Inc.

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FIG. 12 — Effects of prenatal exposure to CrVI on colocalization of Xpnpep2 and Col4 proteins in the ED 17.5 ovaries. On ED 17.5, ovaries from fetuses were processed for whole-mount double immunofluorescence assay. Colocalization of Xpnpep2 and Col4 proteins was evaluated by confocal microscopy. CrVI significantly increased colocalization of Xpnpep2 and Col4 proteins compared to control (overlay; E and F). The width of field for images was 115 μm. Arrowheads indicate localization of Xpnpep2 (A and B), localization of Col4 (C and D), and colocalization of Xpnpep2 and Col4 (E and F) in a GCN. Histogram of colocalization coefficient (G) and negative control (H) are shown. *P < 0.05, control vs. CrVI.