Table 1. Percentage and amount of holo-OCP obtained from 1 L culture of E.coli. The Sequences of amino acids added to the N- or C-terminus are given.
| Name of OCP | Added Amino acid sequence | Total OCP (mg/L) | Holo-OCP (% of total OCP) | Holo-OCP (mg/L) |
|---|---|---|---|---|
| ECN – Synechocystis OCPs | ||||
| Syn-Ctag-ECN | -HHHHHH-StopCodon | 4–6 | 25–40 | 1–5 |
| Syn-pDuet-ECN | MGSS-HHHHHH-SQDP- | 18–22 | 35–45 | 5–10 |
| Syn-MIX15aaCtag-ECN | MGSSRLDNPEKTDIEP- | 20–22 | 30–40 | 5–10 |
| Syn-NC15aaCtag-ECN | MGSSNQANQVTLNPQV- | 18–21 | 45–55 | 8–10 |
| Syn-NC10aaCtag-ECN | MGSSNQANQVT- | 9–11 | >95 | 10 |
| Syn-NC8aaCtag-ECN | MGSSNQANQ- | 15–17 | >95 | 15 |
| Syn-NC6aaCtag-ECN | MGSSNQA- | 19–21 | 78–85 | 15–20 |
| Syn-NC3aaCtag-ECN | MGSS- | 8–10 | 70–80 | 5–10 |
| Syn-C8aaCtag-ECN | MGSSKKRRA- | 20–22 | 78–85 | 15–20 |
| Syn-3aaNtag-ECN | MGSS-HHHHHH- | 30–35 | ≥95 | 30–35 |
| ECN - Anabaena and Arthrospira OCPs | ||||
| Ana-3aaNtag-ECN | MGSS-HHHHHH- | 50–60 | 50–60 | 25–35 |
| Ana-Ctag-ECN | -HHHHHH-StopCodon | 50–60 | 40–45 | 20–25 |
| Arthro-3aaNtag-ECN | MGSS-HHHHHH- | 25–30 | 30–40 | 5–15 |
| Arthro-Ctag-ECN | -HHHHHH-StopCodon | 15–20 | 10–25 | 1–5 |
| CAN - OCPs | ||||
| Arthro-3aaNtag-CAN | MGSS-HHHHHH- | 25–30 | 60–65 | 15–20 |
| Arthro-Ctag-CAN | -HHHHHH-StopCodon | 30–35 | 50–60 | 15–20 |
| Syn-3aaNtag-CAN | MGSS-HHHHHH- | 8–10 | 75–85 | 5–10 |
| Ana-3aaNtag-CAN | MGSS-HHHHHH- | 60–70 | 40–45 | 25–30 |
| Ana-Ctag-CAN | -HHHHHH-StopCodon | 60–70 | 50–60 | 30–45 |