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. 2015 Jan 21;33(4):1599–1608. doi: 10.3892/or.2015.3746

Figure 4.

Figure 4

Effect of endocannabinoids on the cell cycle distribution of PC3 cells and BPH and PrC cultures. Cell cycle analysis was assessed to determine whether the effect on cell viability noted in the MTT assays could be cell cycle-dependent. PC3 cells and primary cultures of BPH and PrC were treated and then labeled. The labeled cells were analyzed using a FACScan cytometer, and the percentage of cells in the G1, S and G2 phases were calculated using the WinMDI v2.8 software (n=3, P>0.05). BPH, benign prostatic hyperplasia tissue; PrC, prostate cancer; C, control; Me, methanandamide; 2-AG, 2-arachidonoyl glycerol; Ana, anandamide.