FIGURE 10.

Role of G protein and PI3K signaling in DEPTOR degradation and Akt signaling promoted by CXCR4. A, role of G protein and PI3K signaling on DEPTOR degradation. HeLa cells were pretreated with DMSO, pertussis toxin (PTX), wortmannin, or 3-MA, followed by treatment with vehicle (VEH) (minus symbol) or CXCL12 (plus symbol) for 3 h. Whole cell lysates were immunoblotted for DEPTOR, CXCR4, and actin. Representative blots from three independent experiments are shown. B–D, role of G protein and PI3K signaling on Akt activation and signaling. HeLa cells were pretreated with pertussis toxin (B, PTX), gallein (C), and varying concentrations of wortmannin or 3-methyladenine (D), followed by treatment with vehicle (VEH), CXCL12, or EGF for 5 min. Equal amounts of whole cell lysates were immunoblotted for the indicated proteins and their phosphorylation status. Arrow represents pAkt-T308 and asterisks represent nonspecific bands (B–D). Representative blots from three independent experiments are shown.