FIGURE 9.

Depletion of ESCRT-I subunit UBAP1 does not have an effect on GPCR and RTK-promoted mTORC1 activity. A–D, HeLa cells were treated with siRNA directed against ESCRT-I subunit UBAP1 and stimulated with CXCL12 (A), norepinephrine (B), EGF (C), or insulin (D) for 5 min. Equal amounts of whole cell lysates were immunoblotted for p70S6K and its phosphorylation state at Thr-389, an mTORC1 phosphorylation site. Samples are from the experiment described in Fig. 4. Representative blots from four independent experiments are shown.