FIGURE 6.
pUL31 disrupts supported lipid bilayers. A, supported lipid bilayers (SLB) mimicking the nuclear envelope with rhodamine-PE (red) form continuous fluid phase. Bilayers are disrupted upon addition of His6-EGFP-pUL31 (green). A few seconds after addition, pUL31 forms 1.2 ± 0.2 μm patches that grow over time until they cover the whole area (around 90 s). The bilayer is destroyed, and lipid aggregation is seen over this period of time. B, addition of His6-EGFP did not change the bilayer. C, particle analysis of the patches showing the growth of the particles over time. After 40 s, particles fuse and can no longer be analyzed. Scale bars = 50 μm.