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Examination of multimer formation by cross-linking different forms of the carboxyl terminus of TcdB. Cross-linking of B2′B3 and B3 was performed with BS3 as described under “Experimental Procedures.” The cross-linked protein was resolved by 3–8% gradient SDS-PAGE (A) or 1.5% SDS-AGE (B) and then stained with Coomassie Blue.
