TABLE 3.

Thin filament activated ATPase assays

Values are mean ± S.E. from 4 to 6 independent assays. Statistical significance was calculated using Student's t test.

Tm	Disease phenotype	pCa50	ΔpCa50 from WT	% maximum activity	% minimum activity	Hill coefficient, nH
WT		6.33 ± 0.09		104 ± 2	8 ± 1	1.63 ± 0.10
E62Q	HCM	6.39 ± 0.06a	+0.06	131 ± 7a	8 ± 1	1.39 ± 0.16
D84N	DCM	6.17 ± 0.03b	−0.16	82 ± 1a	2 ± 1	1.40 ± 0.1b
I172T	HCM	6.51 ± 0.07a	+0.18	109 ± 11	14 ± 3	1.98 ± 0.44
E181K	Predicted HCM	6.78 ± 0.02a	+0.45	150 ± 6b	23 ± 3b	2.12 ± 0.53
L185R	HCM	7.26 ± 0.02b	+0.93	224 ± 5c	32 ± 8a	1.77 ± 0.19a
S215L	HCM	6.49 ± 0.13a	+0.16	152 ± 5a	21 ± 3a	1.11 ± 0.07a
D230N	DCM	6.11 ± 0.16a	−0.22	86 ± 7	10 ± 3	1.85 ± 0.36
M281T	HCM	6.63 ± 0.02a	+0.30	132 ± 5a	9 ± 1	1.76 ± 0.18

^a p < 0.05.

^b p < 0.01.

^c p < 0.005.