FIGURE 2.

SK channel expression in HL-1 cells cultured in NG and HG. A, immunoblots of SK1, SK2, SK3, and GAPDH in HL-1 cells after 2 weeks of culture in medium containing NG (5 mm) and HG (25 mm). B, group results showing that SK2 and SK3 protein expression was significantly down-regulated in HG culture, whereas that of SK1 was not (n = 8). * represents p < 0.05 versus controls. C, group data showing relative mRNA expression of SK1, SK2, and SK3 in HL-1 cells cultured in NG and HG (n = 3). There was no significant difference for SK1 and SK2 mRNA between NG and HG culture, but that of SK3 was increased by more than 6-fold in HG. * represents p < 0.05 versus NG. Error bars represent S.E.