FIGURE 7.

Conditioned medium from KGF-treated MLE15 cells reduces the growth of M. tuberculosis in RAW cells by GM-CSF- and nitric oxide-dependent mechanisms. M. tuberculosis-infected RAW cells were treated with conditioned medium from KGF-treated MLE15 cells (KGF-CM) in the presence or absence of neutralizing antibody to GM-CSF, isotype control antibody, or l-NAME (nitric-oxide synthase inhibitor). After 5 days, the RAW cells were lysed, plated on 7H11 Middlebrook agar, and cultured, and cfu were quantified. Results are shown as the mean ± S.D. (error bars) of quadruplicate samples and are representative of three independent experiments. **, p < 0.05; *, p < 0.001 versus untreated control cells.