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. 2015 Mar 13;6:114. doi: 10.3389/fimmu.2015.00114

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Copyright © 2015 Moalli, Proulx, Schwendener, Detmar, Schlapbach and Stein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamic B cell behavior on TDA-coated FDCs. (A) 2PM image sequence showing B cell migration along tdTom⁺ structures 31–95 μm below the TDLN capsule in CX3CR1^+/gfp recipients. A representative B cell track is shown as dotted line. Scale bar, 10 μm; time in minutes and seconds. (B) Representative tracks (>10 time points) of B cells migrating taken from the image sequence shown in A. One square corresponds to 11.2 μm. (C) Quantification of B cell speeds in TDLNs. (D) Quantification of turning angles of B cells in TDLNs. Data in (A–D) are from three mice in one experiment, with 433 B cell tracks analyzed.