FIGURE 5.

PRN694 does not inhibit FcR-induced macrophage, dendritic cell, or mast cell activation. Monocyte-derived macrophages (A) or dendritic cells (B) were stimulated with immobilized human IgG for 24 h. Supernatant levels of TNF were measured. C, monocyte-derived dendritic cells were stimulated with immobilized human IgG for 24 h. Supernatant levels of IL-12 were measured. A–C, data are derived from three individual experimental donors. E, RBL-2H3 mast cells were coated with anti-DNP IgE and subsequently stimulated with DNP for 30 min. Supernatant levels of histamine were measured via ELISA. F, RBL-2H3 mast cells were coated with anti-DNP IgE and subsequently stimulated with DNP for 30 min. Molecular activation was characterized by immunoblot analysis of pERK1/2. In addition, BTK and ITK expression were tested. Jurkat and Mec-1 cells were included as ITK- and BTK-expressing controls. NS, not significant. Error bars, S.E.