FIGURE 2.

Effect of equol on eIF4G and c-Myc expression in cancer and non-cancer cells. Quiescent cells were treated with vehicle or equol (0–50 μm) for 24 h and equal amounts of lysates were immunoblotted for the indicated proteins. A, c-Myc expression in MDA-MB-435 human metatsatic cancer cells. B and C, eIF4G and c-Myc expression in MCF-10 mammary epithelial cells. Representative immunoblots (B) fold changes of protein expression compared with vehicle control, calculated from the integrated density of bands normalized for actin expression. Results are shown as fold-changes in equol-treated cells relative to vehicle controls (n = 3). An asterisk indicates statistical significance at p ≤ 0.05.