FIGURE 3.

Effect of eIF4GI knockdown on IRES-dependent mRNA translation in response to equol treatment. MDA-MB-435 cells with control or eIF4GI silencing expressing a bicistronic reporter mRNA containing a cap-dependent Renilla luciferase (RLuc) followed by a 5′-UTR containing the p120 catenin IRES driving the firefly luciferase (FLuc), or a control without an IRES. Cells were treated with vehicle or equol for 24 h, lysed, and the relative IRES activity analyzed as 570 nm FLuc/480 nm RLuc from equal amounts of lysate. IRES activity was quantified relative to control activity for vehicle or equol treated cells. Results show fold change in IRES activity compared with vehicle for n = 3 ± S.E. An asterisk indicates statistical significance of p ≤ 0.05.