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. 2015 Jan 20;290(10):6156–6167. doi: 10.1074/jbc.M114.627109

FIGURE 6.

FIGURE 6.

INCENP staining is abnormal in CAP-D3T1403A cells. A, asynchronous CAP-D3WT and CAP-D3T1403A cells were grown in medium with Dox for 48 h and subjected to a hypotonic treatment, then cytospun and fixed with 4% paraformaldehyde. Cells were stained with anti-INCENP antibody (red), with chromosomes counterstained with DAPI (blue). GFP (green) represents CAP-D3 signal. Representative images are shown. Scale bar is 5 μm. B, asynchronous CAP-D3WT and CAP-D3T1403A cells were grown in medium with Dox for 24 h and subjected to a hypotonic treatment, then cytospun and fixed with 4% paraformaldehyde. Cells were stained with anti-CENP-O antibody (red), with chromosomes counterstained with DAPI (blue). GFP (green) represents CAP-D3 signal. Representative images are shown. Scale bar represents 5 μm.