TABLE 3.
PMCA residence time in EP (t(EP)) in the presence of different reconstitution systems
t(EP) is the ratio between EP levels in steady state and the Ca2+-ATPase activity, which were determined in the same experimental conditions (30 μm ATP and 25 °C).
| XPC | EP level | Specific activity | t(EP) |
|---|---|---|---|
| nmol Pi·mg−1 | nmol Pi·mg−1·s−1 | ms | |
| XDMPC = 0.25 | 0.33 ± 0.03 | 68.2 ± 1.9 | 4.5 ± 0.2 |
| XDMPC = 0.70 | 0.47 ± 0.06 | 38.0 ± 1.1 | 11.9 ± 0.8 |
| XDOPC = 0.30 | 0.22 ± 0.01 | 45.6 ± 2.3 | 4.7 ± 0.1 |
| XDOPC = 0.60 | 0.26 ± 0.01 | 28.7 ± 2.1 | 7.9 ± 0.2 |