FIGURE 1.

Expression of miR-520g is correlated with resistance to 5-FU-induced apoptosis in colon cancer cells. A and B, RKO, HCT116, FET, and GEO cells were treated with different concentrations of 5-FU for 72 h. MTT assays (A) and DNA fragmentation assays (B) showed different sensitivity of these cells to 5-FU treatment. C, Western blot analysis showed that cleaved PARP (C-PARP) was higher in RKO and HCT116 cells than in FET and GEO cells after treatment with 10 μg/ml 5-FU for 72 h. Ctr, control. D, FET and GEO cells expressed much higher levels of miR-520g compared with RKO and HCT116 cells as determined by real-time qPCR analysis. The data are presented as the mean ± S.D. of triplicate experiments.