FIGURE 5.

A miR-520g inhibitor sensitizes FET and GEO cells to 5-FU-induced apoptosis. A chemically synthesized miR-520g inhibitor (Inhib) was transfected into FET and GEO cells, resulting in reduced miR-520g expression as determined by real-time qPCR assays (A) and increased p21 expression as determined by Western blot analysis (B). Ctr, control. The miR-520g inhibitor-transfected cells were treated with different concentrations of 5-FU for 72 h. MTT assays (C) and DNA fragmentation assays (D) showed that the inhibitor sensitized FET and GEO cells to 5-FU-induced apoptosis. The data are presented as the mean ± S.D. of triplicate experiments. ***, p < 0.001.