FIGURE 2.

ZFP407 does not regulate GLUT4 protein trafficking. Differentiated 3T3-L1 adipocytes stably expressing myc-GLUT4-eGFP were electroporated with control siRNA, Exo84 siRNA, or Zfp407 siRNA. Four days later, the cells were serum starved and treated with 100 nm insulin. A and B, cells were fixed without permeabilizing. The cells were incubated with anti-myc secondary antibody overnight. GFP and MYC localization were determined by confocal microscopy. A, GFP-GLUT4 positive cells were blindly counted as either positive or negative for plasma membrane localized GFP. B, plasma membrane positive cells were then blindly counted as either positive or negative for MYC staining. Mean ± 95% confidence intervals are shown. n = 95, 84, 71, and 60 cells, respectively.