FIGURE 8.

Regulation of Glut4 by ZFP407 is mediated by PPARγ. A, Glut4 mRNA levels were analyzed by qPCR in 3T3-L1 adipocytes electroporated with control or Zfp407 siRNA followed by treatment with 1 μm ROSI or dimethyl sulfoxide (DMSO). B, a PPARγ consensus reporter vector was co-electroporated in 293T cells with the following indicated vectors: pRK5-myc (empty vector control), Zfp407 cDNA, and PPARγ cDNA. C, levels of total Glut4 and relative Glut4 transcript variants were evaluated by qRT-PCR and compared with total Glut4 transcript levels to calculate the fold-change for each variant as compared with the correctly spliced transcripts. Total RNA was obtained from 3T3-L1 adipocytes treated for 24 h with ROSI (1 μm). Data are expressed as mean ± S.E. * indicates p < 0.05; # indicates p < 0.001; and ## indicates p < 0.0001.