FIGURE 6.

The plastid-located MORF2 and MORF9 proteins form homo- and heteromers and also interact with the dual-targeted MORF5 and MORF8 proteins. The comparative summary of the results from the three assay systems, the Y2H experiments (Fig. 3), the pulldown assays (PD; Fig. 4), and the BiFC complementations (Fig. 5), shows that the plastid-located MORF proteins connect to homo- and heteromers. Orientations of the respective assays are indicated with letters, positive signals are represented by a plus sign and shaded, and a minus sign indicates that no signal is seen. All interactions are detected in at least two of the three assays, and only MORF2-MORF5 and MORF8-MORF9 are seen in only one assay system. n.d. = not determined due to problems encountered with expression of the respective recombinant proteins.