FIGURE 7.

The interaction network between the mitochondrial MORF proteins. Interactions between the mitochondrially located MORF proteins 1, 3, 4, 6, and 7 and the dual-targeted MORF5 and MORF8 proteins were probed in Y2H (Fig. 3), pulldown (PD; Fig. 4), and BiFC analyses (Fig. 5). The mitochondrial MORF1 protein and the dual-targeted MORF8 protein contact all other MORFs in at least two of the three assay systems. MORF3, which is (like MORF1 and MORF8) involved in numerous RNA editing events, also contacts most other MORFs and forms heterodimers with MORF1, MORF4, MORF5, and MORF8 and in the pulldown assays, also with MORF6 and MORF7. The proteins involved in fewer editing events, MORF4, MORF6, and MORF7, form selected heterodimers mostly with MORF1 and a few other MORFs. n.d. = not determined due to problems encountered with expression of the respective recombinant proteins.