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. 2015 Jan 13;290(10):6495–6506. doi: 10.1074/jbc.M114.592717

FIGURE 7.

FIGURE 7.

The C- and E-loop of Duox are not associated with O2⨪ leakage. A, alignment of the C-loops of Duox1 and Duox2 and illustrations of chimeric Duox2 mutants indicating the residue and number of amino acids changes in the C-loop from Duox2 to Duox1 (Duox2→1(C)). B, Duox2→1(C:10aa) and DuoxA pairs were transfected into HEK293 cells. O2⨪ and ROS were measured by chemiluminescence assays using Diogenes and luminol + HRP, respectively. The Duox2→1(C:10aa) + DuoxA pairs show no decreased O2⨪ production. Immunoblotting (from the same membrane and image) detects comparable expression levels of various Duox and DuoxA pairs. C, alignment of the E-loops of Duox1 and Duox2 and illustrations of chimeric Duox2 mutants indicating the residues change in the E-loops from Duox2 to Duox1 (Duox2→1(E)). D, Duox2→1(E:TY/RF) and DuoxAs pairs were transfected into HEK293 cells. O2⨪ and ROS were measured by chemiluminescence assay using Diogenes and luminol + HRP, respectively. The Duox2→1(E:TY/RF) + DuoxA pairs show no decreased O2⨪ production. Immunoblotting detects comparable expression levels of various Duox and DuoxA pairs.