FIGURE 8.
The A-loops of both Duox1 and Duox2 prevent O2⨪ leakage. The illustrations indicate changes in the core aa sequences of the A-loops (7 aa) from Duoxes to Nox (1 or 5). Green and purple colors indicate the changes from Duox2 to Nox1 or Nox5 (Duox2→Nox(A)) and those from Duox1 to Nox1 or Nox5 (Duox1→Nox(A)), respectively. Various pairs were transfected into HEK293 cells. O2⨪ was measured by a chemiluminescence assay using Diogenes. Immunoblotting detects comparable expression levels of various Duox and DuoxA pairs.