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. 2015 Jan 17;290(10):6507–6515. doi: 10.1074/jbc.M114.620369

FIGURE 2.

FIGURE 2.

The effect of nuclear receptor ligands on miR-22 expression. A, ligands for nuclear receptors including rifampicin (10 μm), TCPOBOP (250 nm), WY14643 (100 μm), CDCA (100 μm), vitamin D3 (0.1 μm), and all-trans-retinoic acid (10 μm) were used to treat Huh7 and HCT116 cell lines for 24 h. B, expression of miR-22 along with SHP and FGF19 were quantified after 24 h of CDCA treatment (50, 100, and 150 μm). C, expression levels of miR-22 along with SHP and FGF19 RNA were quantified at the indicated times in CDCA-treated cells (150 μm). D, expression of miR-22 was quantified after 24 h treatment of LCA (20 μm), CDCA (50 μm), DCA (50 μm), CA (50 μm), and hyodeoxycholic acid (HDCA, 50 μm). E, expression levels of miR-22 along with SHP and FGF19 mRNA were quantified after 24 h of GW4064 treatment (5 μm). The data are presented as the means ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001.