FIGURE 5.

Repression of CCNA2 by miR-22 at the mRNA and protein level. A, expression of miR-22 and CCNA2 were quantified in Huh7 and HCT116 cells 48 h after transfection with either miR-22 mimics or scramble controls. B, protein level of CCNA2 was determined by Western blot. C, expression of miR-22 and CCNA2 were quantified in CDCA-treated Huh7 and HCT116 cells 48 h after transfection of miR-22 inhibitors or scramble controls. D, protein level of CCNA2 was determined by Western blot. E, cell cycle distribution of miR-22 mimic transfected-Huh7 or HCT116 cells was measured by flow cytometry after 72 h transfection. The data are presented as the means ± S.D. *, p < 0.05; ***, p < 0.001.