TABLE 2.

Collagen gene expression: interaction of gene and diet

Total RNA was extracted from the livers of 5-h fasted mice and reverse-transcribed into cDNA. qPCR was used to determine gene expression of several collagen chains (n = 5–7/group). Data are represented as means ± S.E.

Collagen	Chow		High fat
	itga1+/+	itga1−/−	itga1+/+	itga1−/−
α1(I)	1.1 ± 0.2	3.0 ± 0.6a	1.5 ± 0.1a	4.2 ± 1.2b
α2(I)	1.1 ± 0.3	9.6 ± 3.0a	2.6 ± 0.6a	13.6 ± 5.1b
α1(III)	1.2 ± 0.2	4.0 ± 0.6a	1.1 ± 0.3	4.5 ± 1.6b
α1(IV)	1.0 ± 0.1	2.3 ± 0.4a	1.8 ± 0.5	3.4 ± 1.0

^a p < 0.05 compared with chow-fed itga1^+/+ mice.

^b p < 0.05 compared with HF fed itga1^+/+ mice.