FIGURE 1.

Interaction of the NoLS of PHF6 with RBBP4. A, schematic diagram of PHF6, including two extended plant homeodomain (PHD) zinc-finger domains and four NLSs (NSL1–NLS4). The sequence of the putative NoLS (composed of NLS3 and NLS4) is shown below in italic type, and the NLS3 and NLS4 sequences are underlined. aa, amino acids. B, determination of the affinity of the PHF6(157–171) peptide for the RBBP4 protein using ITC. Data were fitted to a one-site binding model using Origin 7, and calculated binding parameters were ΔH = −15,180 ± 225.2, and ΔS = −27.5.