Table 2.
Antimicrobial properties of known and potential new antimicrobial peptides in Tribolium castaneum.
| Gene ID | Molecular weight (kDa) | Peptide length (AA) | Hydrophobic ratio | Net charge | Glycine content | Proline content | Fold change wild-type | Fold change control |
|---|---|---|---|---|---|---|---|---|
| Cecropin1/TC000499 | 3.67 | 31 | 58% | +5 | 6% | 0% | Inf | Inf |
| Cecropin3/TC000500 | 9.80 | 90 | 43% | +2 | 6% | 13% | Inf | 49x |
| attacin2/TC007738 | 15.80 | 145 | 37% | +7 | 12% | 4% | 3098x | 2190x |
| Coleoptericin1/TC005093 | 15.99 | 141 | 30% | −1 | 9% | 7% | 2392x | 18067x |
| Defensin2/TC010517 | 8.73 | 79 | 50% | +6 | 6% | 1% | 1183x | Inf |
| Defensin3/TC012469 | 9.42 | 83 | 50% | +7 | 3% | 1% | 908x | Inf |
| attacin1/TC007737 | 17.49 | 165 | 28% | +9 | 18% | 3% | 869x | 3696x |
| TC007858 | 20.14 | 182 | 35% | 0 | 11% | 3% | 484x | 54x |
| Defensin1/TC006250 | 14.91 | 132 | 46% | +11 | 4% | 3% | 187x | 1551x |
| TC011036 | 12.89 | 109 | 39% | +13 | 2% | 6% | 138x | 11x |
| Coleoptericin2/TC005096 | 15.96 | 141 | 30% | −1 | 9% | 7% | 91x | 227x |
| TC015479 | 13.00 | 120 | 42% | +6 | 5% | 1% | 80x | 26x |
| TC007763 | 16.87 | 158 | 37% | +4 | 6% | 17% | 47x | 67x |
| TC004646 | 15.04 | 135 | 34% | +2 | 7% | 7% | 40x | 29x |
| TC008806 | 15.83 | 142 | 33% | +2 | 10% | 2% | 31x | 37x |
| TC009336 | 13.50 | 137 | 30% | −4 | 39% | 2% | 15x | 7x |
| TC014565 | 20.73 | 176 | 38% | +17 | 2% | 2% | 14x | 9x |
| TC001030 | 14.62 | 137 | 29% | +9 | 10% | 12% | 9x | 16x |
| TC001784 | 13.54 | 150 | 27% | +7 | 43% | 2% | 8x | 6x |
| TC005478 | 13.70 | 122 | 45% | +10 | 4% | 1% | 6x | 7x |
| TC015612 | 20.32 | 182 | 36% | +7 | 6% | 6% | 6x | 2x |
| TC007901 | 7.25 | 64 | 25% | +5 | 7% | 10% | 6x | 5x |
| TC015304 | 19.30 | 180 | 38% | +2 | 6% | 9% | 5x | no hit |
| TC011733 | 11.89 | 106 | 46% | +3 | 2% | 0% | 5x | 5x |
| TC003374 | 12.22 | 124 | 61% | +3 | 1% | 9% | 2x | 9x |
| TC008557 | 17.82 | 172 | 31% | +2 | 18% | 0% | 3x | 5x |
| TC015754 | 15.69 | 140 | 34% | +5 | 4% | 7% | 2x | 2x |
| TC000435 | 11.84 | 105 | 37% | +5 | 5% | 0% | 2x | 2x |
| TC009096 | 12.84 | 111 | 16% | +16 | 9% | 6% | 2x | 2x |
In the table are known antimicrobial peptides and those proteins that show at least a twofold induction upon infection, they are smaller than 200 amino acids and are not negatively charged. TC009336 was included because of the high glycine content.