Figure 3.

Structure–function analysis and identification of Unkempt as an RBP. (A) A series of deletion mutants of Unkempt protein examined for their capacity to impact cellular morphology. Internal deletions are indicated by bracketed regions. (B) The morphologies of HeLa cells inducibly expressing GFP alone or GFP and either of the indicated Unkempt mutants were quantified by calculating their axial ratios (see Supplemental Fig. S2K). The results are compared with GFP control. (*) P = 0.0007; (**) P < 0.0001. (C) Outline of the Unkempt iCLIP experiment coupled with deep sequencing (see also Supplemental Table S1). (D) Binding of Unkempt to RNA in SH-SY5Y cells. (Top) Autoradiogram. The bottom three stripes are immunoblots of UNK and Actin. (WT) Wild-type; (KD) knockdown.