Figure 4.

Unkempt-bound RNA species and the mode of RNA binding. (A) Distribution of Unkempt iCLIP tag clusters among different RNA segments in SH-SY5Y cells. Similar distributions of clusters were observed in HeLa cells and embryonic brains (data not shown). (B) Metatranscript analysis showing the positional frequency of Unkempt binding sites along the length of all target mRNAs in SH-SY5Y cells. (C) Gene ontology (GO) analysis of Unkempt target transcripts in SH-SY5Y cells. The top 10 GO terms are ranked by their P-values. (see also Supplemental Table S3). (D) Unkempt contacts its target transcripts commonly at just one dominant binding site. Snapshots from the University of California at Santa Cruz Genome Browser (http://genome.ucsc.edu; human assembly GRCh37/hg19 and mouse assembly GRCm38/mm10; Kent et al. 2002) of different Unkempt target genes depict binding positions in the indicated sample types (see also Supplemental Fig. S6A).