Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 1;29(5):538–554. doi: 10.1101/gad.252767.114

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — VL30 reactivation is associated with partial loss of DNA methylation. (A) Bisulfite sequencing analysis of VL30-pro ERVs in Zfp809^GT MEFs. Full and empty circles represent methylated and unmethylated CpG sites, respectively. (B) qMSP analysis of Zfp809^GT MEFs. Graphs show the genomic occurrence of unmethylated VL30-pro elements as percentage of methylated VL30-pro elements (n = 3 technical replicates). Error bars represent SD. (C) qMSP analysis was performed as above but with genomic DNA isolated from tissues of adult wild-type and Zfp809^GT/GT mice. (D) qMSP analysis of MEF and ESC DNA after ChIP with the indicated antibodies. Graphs show the occurrence of unmethylated VL30-pro elements as percentage of methylated VL30-pro elements (n = 3 technical replicates). Error bars represent SD. (E) Dual-luciferase assay of Zfp809^GT/+ MEFs transfected with a single unmethylated or in vitro CpG methylated VL30-pro LTR (+PBS-pro) cloned into a CpG-free luciferase vector. LTR activity was normalized to CMV promoter activity of a cotransfected renilla luciferase plasmid and is displayed in arbitrary units (AU). Assay was performed in triplicates. Error bars represent SD.