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. 2015 Jan 15;8(1):58–72.

Figure 7.

Figure 7

PM2.5-mediated autophagy is p53-independant. Representative images are shown in the left panels, and quantification relative to β-actin expression (mean ± SD of at least 3 separate experiments) is shown in the right panels. A. PM2.5 activates p53 in A549 cells. Cells were treated with 100 μg/ml PM2.5 for variable periods (0, 6, 12 and 24 h). p53 and p-p53 expression was assessed by western blot analysis. B. p53 inhibition does not affect PM2.5 mediated autophagy. Cells were pretreated for 6 h with p53 inhibitor PFT-α, and then treated with 100 μg/ml PM2.5 for 24 h. p53, p-p53 and LC3 expression was assessed by western blot analysis, *P < 0.05, significant compared to controls or without treatment of PFT-α.