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. 2015 Jan 15;8(1):642–649.

Figure 3.

Figure 3

ADRB1 is a target gene of miR-19a. A-C. Since the 3’UTR of ADRB1 contains three putative miR-19a target sites, we divided them into three segments and subcloned into pGL3 vector independently. HEK293T cells were co-transfected with miRNA control, miR-19a mimic, anti-miR control or miR-19a inhibitor and pGL3-ADRB1 for dual-luciferase assay. PRL-TK containing Renilla luciferase was co-transfected for data normalization. D. M utation analysis of the miR-19a binding site. When 3 nucleotides of the binding site of miR-19a in the 3’-UTR of ADRB1 was mutated, the luciferase activity was not significantly changed by miR-19a mimic. E. ADRB1 protein level in miR-19a mimic or inhibitor-treated HEK293T cells was detected by western blot. The results were analyzed by student t-test, and P<0.05 was considered statistically significant. *P<0.05, compared with control. **P<0.01, compared with control.