Figure 2.

SIRT1 and Phospho-SIRT1 were detected by WB. Mann-Whitney U test was used to analysis expression differences of SIRT1 or Phospho-SIRT1 in cancer tissues and normal tissues. A. Tumor tissues and normal tissues were both extracted for whole protein, cytoplasmic protein and nuclear protein (T = tumor tissue, N = normal tissue). Cytoplasm protein was normalized with β-actin and nucleus protein was normalized with H3. SIRT1 and Phospho-SIRT1 were both localized in the nucleus of cancer tissues and normal tissues. B. Relative expression of SIRT1 and Phospho-SIRT1 in tumor tissues and normal tissues. SIRT1 expression in cancer tissues and normal tissues were 1.351 ± 0.020 and 0.825 ± 0.015, respectively (P < 0.05), Phospho-SIRT1 expression were 0.747 ± 0.022 and 0.381 ± 0.0148, respectively (P < 0.05).