Table 2.
Relative expression of SIRT1 mRNA in tumor tissues and normal tissue
| Tissue | Avg. SIRT1 CT | Avg. GAPDH CT | ΔCT | ΔΔCT | Normalized SIRT1 amount relative to normal 2-ΔΔCt |
|---|---|---|---|---|---|
| Tumor | 27.187 ± 0.165 | 25.265 ± 0.287 | 1.922 ± 0.259 | 0.178 ± 0.259 | 1.1 (0.7-1.1) |
| Normal | 26.725 ± 0.119 | 24.981 ± 0.321 | 1.744 ± 0.299 | 0.000 ± 0.299 | 1.0 (0.8-1.2) |
The comparative CT method was used to analyze the expression of SIRT1 mRNA. Avg., Average; ΔCT = Avg; SIRT1 CT - Avg; GAPDH CT; ΔΔCT = Avg; ΔCT,Tumor - Avg. ΔCT,Normal. Fold change = 2-ΔΔCt.