Table 1.

PCR primers used for E. coli virulence genes amplification

Targets	Primer	Oligonucleotide sequence (5’-3’)	Amplicon size (bp)	Reference
iucD	iucD-F	ACAAAAAGTTCTATCGCTTCC	714	[17]
	iucD-R	CCTGATCCAGATGATGCTC
irp2	irp2-F	AAGGATTCGCTGTTACCGGAC	280	[18]
	irp2-R	TCGTCGGGCAGCGTTTCTTCT
papC	papC-F	TGATATCACGCAGTCAGTAGC	501	[19]
	papC-R	CCGGCCATATTCACATAA
iss	iss-F	ATCACATAGGATTCTGCCG	309	[17]
	iss-R	CAGCGGAGTATAGATGCCA
tsh	tsh-F	ACTATTCTCTGCAGGAAGTC	824	[17]
	tsh-R	CTTCCGATGTTCTGAACGT
vat	vat-F	TCCTGGGACATAATGGTCAG	1000	[17]
	vat-R	GTGTCAGAACGGAATTGT
cva	cva-F	TGGTAGAATGTGCCAGAGCAAG	1200	[17]
	cva-R	GAGCTGTTTGTAGCGAAGCC