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Information of rat Aggrecan and Col2a1 primers for Real-time PCR
| Gene | Accession No. | Sequence (5’-3’) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| Aggrecan | NM_022190.1 | For: TCCACATCAGAAGAGCCATAC | 196 | 62 |
| Rev: AGTCAAGGTCGCCAGAGG | ||||
| Col2a1 | NM_053304 | For: GCTGTGGAAGTGGATGAAGA | 174 | 62 |
| Rev: TGAGGAACTGTGGAGAGACG | ||||
| Gapdh | NM_017008.4 | For: TGAGGACCAGGTTGTCTCCT | 162 | 60 |
| Rev: ATGTAGGCCATGAGGTCCAC |
