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. 2015 Mar 3;3:e817. doi: 10.7717/peerj.817

Figure 5. Leaf hardness phenotype of OxSUB1A and OxSUB1C transgenics.

Figure 5

(A) Hardness comparison of rosette leaves from Arabidopsis Col-0 and plants expressing rice SUB1A-1 and SUB1C-1 genes was determined by a fracture resistance test on leaves of 23-day-old plants. Different letters indicate significant differences from Col-0 (P < 0.01, Student’s t test). Values are means of n = 7 to 13 plants. Error bars are ±S.D. (B–C) Transcript accumulation in 7-day-old complete seedlings at ZT8 (middle of the day) of Col-0, OxSUB1A and OxSUB1C transgenics. (A) BGL2 transcript, (B) AtEXP2 transcript. Transcript abundance was determined by quantitative RT-PCR and normalized to abundance in Col-0 using TUBULIN2 as reference. Values are means of two independent experiments with three technical replicates each. Different letters indicate significant difference with Col-0 (P < 0.01, Student’s t test). Values are means ± S.E.