Table 1. Free reducing sugars, cell wall digestibility and starch content of 22-day-old rosettes of Arabidopsis Col-0 and transgenics expressing rice SUB1A-1 or SUB1C-1.
| Free reducing sugara
(mg of reducing sugars g−1 FW)/% of WT |
Cell wall digestibilityb
(mg of glucose g−1 FW)/ % of WT |
Starch contentc
(mg of glucose g−1 FW)/ % of WT |
|
|---|---|---|---|
| Col-0 WTd | 0.40 ± 0.01 a | 0.100 ± 0.004 a | 8.5 ± 2.7 a |
| OxSUB1A L5 | 0.54 ± 0.02/+37 b | 0.116 ± 0.008/+16 b | 15.9 ± 0.7/+88 b |
| OxSUB1A L12 | 0.44 ± 0.03/+10 a | 0.123 ± 0.010/+23 b | 11.6 ± 0.8/+36 c |
| OxSUB1C L6 | 0.42 ± 0.02/+6 a | 0.101 ± 0.012/+1 a | 10.6 ± 1.1/+25 ac |
| OxSUB1C L10 | 0.33 ± 0.01/−17 c | 0.062 ± 0.005/−38 c | 7.05 ± 0.5/−7 d |
Notes.
a
As measured by Miller’s reagent (dinitrosalicylic acid).
b
After 24 h saccharification with Accellerase enzyme mix.
c
After amylase/amyloglucosidase digestion.
d
Different letters indicate a significant difference between genotypes (P < 0.05, means comparison, Student’s t test). Values are means ± S.E. of three independent experimental replicates, each with n = 5 plants.