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. 2015 Feb 4;593(Pt 5):1169–1181. doi: 10.1113/jphysiol.2014.285148

Figure 1.

Figure 1

Transcriptional expression of protease-activated receptors (PARs) in murine colonic smooth muscles

A, representative agarose end-point gel of RT-PCR products revealed F2r (195 bp), F2rl1 (151 bp) and F2rl2 (139 bp) expression in unsorted cells after enzymatic dispersion of the tunica muscularis of the colon, sorted smooth muscle cells (SMC), sorted interstitial cells of Cajal (ICC) and sorted platelet-derived growth factor receptor α (PDGFRα+) cells. B, summary data from real-time quantitative PCR analysis of PARs from unsorted and sorted cells (n = 4) showing relative expression of PAR isoforms. Inset graph has expanded y-axis to show the levels of the relatively low expression of PAR genes in SMCs and ICC.